{"id":2553,"date":"2022-02-03T07:05:49","date_gmt":"2022-02-03T06:05:49","guid":{"rendered":"https:\/\/toulouse-sgencfdt.fr\/WORDPRESS_ITRF\/?p=2553"},"modified":"2022-02-03T07:05:49","modified_gmt":"2022-02-03T06:05:49","slug":"mes-recettes-de-labo","status":"publish","type":"post","link":"https:\/\/toulouse-sgencfdt.fr\/WORDPRESS_ITRF\/2022\/02\/03\/mes-recettes-de-labo\/","title":{"rendered":"Mes recettes de LABO"},"content":{"rendered":"<p>Pour pr\u00e9parer certains r\u00e9actifs, on a tous nos recettes&#8230; Voici les miennes:<\/p>\n<ul>\n<li><span style=\"color: #333399;\"><strong>Liqueur de Fehling: (forme un pr\u00e9cipit\u00e9 rouge brique en pr\u00e9sence d&#8217;ald\u00e9hyde, test effectu\u00e9 en bain marie)\u00a0<\/strong><\/span>\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li>solution A: 40g de CuSO4,5H2O dans une fiole jaug\u00e9e d&#8217;1L compl\u00e9t\u00e9e \u00e0 l&#8217;ED<\/li>\n<li>solution B: 200g de Sel de Seignette (potassium sodium tartrate) + 150g de NaOH, dans une fiole jaug\u00e9e d&#8217;1L compl\u00e9t\u00e9e \u00e0 l&#8217;ED<\/li>\n<li>Au moment du TP, m\u00e9langer \u00e0 volume \u00e9gale la solution A avec la solution B. Vous obtenez la liqueur de Fehling (elle se conserve quelques temps)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<ul>\n<li><span style=\"color: #333399;\"><strong>R\u00e9actif de Biuret (devient violet en pr\u00e9sence de peptide)<\/strong><\/span>:\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li>Dissoudre 3g de CuSO4,5H20 avec 9g de Potassium Sodium Tartrate dans 500mL d&#8217;ED<\/li>\n<li>Ajouter 8g de NaOH et 5g de KI. Compl\u00e9ter \u00e0 1L d&#8217;ED<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<ul>\n<li><span style=\"color: #333399;\"><strong>DNPH<\/strong><\/span>: <span style=\"color: #333399;\"><strong>(formation pr\u00e9cipit\u00e9 rouge rang\u00e9 en pr\u00e9sence d&#8217;ald\u00e9hyde et c\u00e9tone)<\/strong><\/span>\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li>Dans un bain d&#8217;eau glac\u00e9e, dissoudre 15g de DNPH dans 75mL d&#8217;H2SO4 \u00e0 98%<\/li>\n<li>Ajouter 100mL d&#8217;ED<\/li>\n<li>Ajouter 350mL d&#8217;\u00e9thanol.<\/li>\n<li>Attendre que le m\u00e9lange ait refroidi avant de tester votre DNPH<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<li><strong><span style=\"color: #ff6600;\">OU (recette diff\u00e9rente mais qui donne un pr\u00e9cipit\u00e9 jaune)<\/span><\/strong>\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li>Dans un bain d&#8217;eau glac\u00e9e, dissoudre 4g de DNPH dans 20mL d&#8217;H2SO4 \u00e0 98%<\/li>\n<li>ajouter 30mL d&#8217;ED en goutte \u00e0 goutte<\/li>\n<li>ajouter 100mL d&#8217;\u00e9thanol<\/li>\n<li>Attendre que le m\u00e9lange ait refroidi avant de tester votre DNPH<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<ul>\n<li><strong><span style=\"color: #333399;\">Ph\u00e9nolphtal\u00e9ine:<\/span><\/strong>\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li>dissoudre 1g de ph\u00e9nolphtal\u00e9ine dans 100mL d&#8217;\u00e9thanol<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<ul>\n<li><span style=\"color: #333399;\"><strong>Bleu de Thymol:<\/strong><\/span>\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li>dissoudre 0,4g de bleu de thymol dans 1L d&#8217;\u00e9thanol<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<ul>\n<li><strong><span style=\"color: #333399;\">BBT (Bleu de bromothymol)<\/span><\/strong>\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li>dans une fiole jaug\u00e9e 100mL: 0,4 g de BBT dans 20mL d&#8217;\u00e9thanol<\/li>\n<li>ajouter 8mL de NaOH \u00e0 0,1mol\/L<\/li>\n<li>compl\u00e9ter \u00e0 l&#8217;ED<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<ul>\n<li><strong><span style=\"color: #333399;\">H\u00e9lianthine:<\/span><\/strong>\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li>dans 100mL d&#8217;ED bouillante, dissoudre 22mg d&#8217;h\u00e9lianthine<\/li>\n<li>Ajouter 67mL d&#8217;HCl \u00e0 0,1mol\/L<\/li>\n<li>Laisser d\u00e9canter et filtrer<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<ul>\n<li><strong><span style=\"color: #333399;\">Rouge de cr\u00e9sol<\/span><\/strong>\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li>dans une fiole jaug\u00e9e 1L, dissoudre 80mg d&#8217;hydrog\u00e9nocarbonate de Na avec 70mg de KCl et 1g de rouge de cr\u00e9sol en poudre<\/li>\n<li>compl\u00e9ter \u00e0 l&#8217;ED<\/li>\n<li>laisser agiter toute une nuit dans la fiole bouch\u00e9e<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<ul>\n<li><span style=\"color: #333399;\"><strong>Rouge de m\u00e9thyle:<\/strong><\/span>\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li>dissoudre 20mg de rouge de m\u00e9thyl dans 100mL d&#8217;ED chaude<\/li>\n<li>laisser refroidir d\u00e9canter et filtrer<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<ul>\n<li><strong><span style=\"color: #333399;\">Luminol:<\/span><\/strong>\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li>solution A: dissoudre 1g luminol avec 10g de NaOH dans une fiole jaug\u00e9e de 500mL \u00e0 compl\u00e9ter \u00e0 l&#8217;ED<\/li>\n<li>Solution B: dissoudre 15g de ferricyanure de K avec 0,5mL d&#8217;H2O2 \u00e0 30% dans une fiole jaug\u00e9e 500mL compl\u00e9t\u00e9e \u00e0 l&#8217;ED<\/li>\n<li>dans l&#8217;obscurit\u00e9 m\u00e9langer les solutions A et B dans les m\u00eames proportions<\/li>\n<li>Si de la lumi\u00e8re appara\u00eet rajouter un peu de solution B<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Pour pr\u00e9parer certains r\u00e9actifs, on a tous nos recettes&#8230; Voici les miennes: Liqueur de Fehling: (forme un pr\u00e9cipit\u00e9 rouge brique en pr\u00e9sence d&#8217;ald\u00e9hyde, test effectu\u00e9 en bain marie)\u00a0 solution A: 40g de CuSO4,5H2O dans une fiole jaug\u00e9e d&#8217;1L compl\u00e9t\u00e9e \u00e0 l&#8217;ED solution B: 200g de Sel de Seignette (potassium sodium tartrate) + 150g de NaOH, &hellip; <a href=\"https:\/\/toulouse-sgencfdt.fr\/WORDPRESS_ITRF\/2022\/02\/03\/mes-recettes-de-labo\/\" class=\"more-link\">Continuer la lecture de <span class=\"screen-reader-text\">Mes recettes de LABO<\/span>  <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":11,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[26,7,23],"tags":[],"class_list":["post-2553","post","type-post","status-publish","format-standard","hentry","category-generalites","category-securite-au-labo","category-tp-et-calculs"],"_links":{"self":[{"href":"https:\/\/toulouse-sgencfdt.fr\/WORDPRESS_ITRF\/wp-json\/wp\/v2\/posts\/2553","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/toulouse-sgencfdt.fr\/WORDPRESS_ITRF\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/toulouse-sgencfdt.fr\/WORDPRESS_ITRF\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/toulouse-sgencfdt.fr\/WORDPRESS_ITRF\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/toulouse-sgencfdt.fr\/WORDPRESS_ITRF\/wp-json\/wp\/v2\/comments?post=2553"}],"version-history":[{"count":1,"href":"https:\/\/toulouse-sgencfdt.fr\/WORDPRESS_ITRF\/wp-json\/wp\/v2\/posts\/2553\/revisions"}],"predecessor-version":[{"id":2554,"href":"https:\/\/toulouse-sgencfdt.fr\/WORDPRESS_ITRF\/wp-json\/wp\/v2\/posts\/2553\/revisions\/2554"}],"wp:attachment":[{"href":"https:\/\/toulouse-sgencfdt.fr\/WORDPRESS_ITRF\/wp-json\/wp\/v2\/media?parent=2553"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/toulouse-sgencfdt.fr\/WORDPRESS_ITRF\/wp-json\/wp\/v2\/categories?post=2553"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/toulouse-sgencfdt.fr\/WORDPRESS_ITRF\/wp-json\/wp\/v2\/tags?post=2553"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}